Scientific Publications
Peer-reviewed research using Nanolive imagingDiscover a list of peer-reviewed scientific publications using Nanolive imaging in the fields of drug discovery, cell metabolism, etc.
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The antimicrobial peptide tilapia piscidin 3 induces mitochondria-modulated intrinsic apoptosis of osteosarcoma cells
Authors: Chien-HanYuan, Yi-LingMa, Po-ChangShih, Chao-TingChen, Shu-YuCheng, Chieh-YuPan, Yen-HsuanJean, Yih-MinChu, Sung-ChunLin, Yu-ChengLai, Hsiao-MeiKuo
DOI: https://doi.org/10.21873/anticanres.14232, 2020
Published in: Biochemical Pharmacology
Tags: Immunooncology, oncology, cell metabolism, OS MG63, osteosarcoma, intrinsic apoptosis, TP3, antimicrobial peptide, mitochondria, caspase, blebbing, shrinkage, nuclear fragmentation, chromatin condensation, apoptopodia, ROS, acidification, fission, fusion
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Feature Application: Characterization of stem cells
This Feature Application shows the enormous potential of Nanolive technology for observing stem cells in 3D for long periods of time, at unprecedented spatial and temporal resolution (case study 1). We use our technology to capture all the phases of mitosis (case study 2) and the dynamics of stress fiber formation (case study 3), in unparalleled detail. We then showcase our most ground-breaking contribution to this research field, monitoring the sub-cellular morphological changes that occur during stem cell differentiation. We focus on neural differentiation at the single cell (case study 4) and population level (case study 5), before examining the temporal evolution of osteogenic (case study 6) and chrondrogenic differentiation (case study 7) in high resolution.
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Feature Application: Characterizing cell metabolism
This Feature Application highlights the huge potential of Nanolive cell imaging for metabolic studies. We begin by proving that mitochondria and LDs have specific RI signals that allow them to be identified and mapped, in 3D (case study 1). We show that it is possible to image fission-fusion dynamics in mitochondria (case study 2), induce and detect mitochondrial dysfunction (case study 3) and then restore mitochondrial function by the addition of a specific drug (case study 4). Finally, we show that it is possible to quantify LD dynamics in unperturbed (case study 5) and drug-perturbed conditions (case study 6).
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Feature Application: Characterising cancer cells and their interactions with immune cells
In this application note, we begin by examining the interactions between t-cells, dendritic cells, and macrophages (case study 1). We then image t-cells responses to cancer cells (case study 2) and the interactions that occur between t-cells and macrophages (case study 3). We show Nanolive imaging can be used to monitor the response of cancer cells to promising antibody drug conjugate (ADCs) products (case study 4) and novel bispecific antibodies (case studies 5 and 6). Finally, we finish with an example of a drug screen where we describe the phenotypic response of living cells to various kinase inhibitors that have shown promise as anti-cancer treatments (case study 7).
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BA6 Induces Apoptosis via Stimulation of Reactive Oxygen Species and Inhibition of Oxidative Phosphorylation in Human Lung Cancer Cells
Authors: Lianna Y. D’Brant, Habben Desta, Ting Chean Khoo, Anna V. Sharikova, Supriya D. Mahajan, Alexander Khmaladze
DOI: https://doi.org/10.1117/1.JBO.24.4.046503, 2019
Published in: Journal of Biomedical Optics
Tags: Single cell characterization, drug discovery, neuroscience, rat, C6 glial cells, methamphetamine, Raman spectroscopy, apoptosis
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3PO as a Selective Inhibitor of 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 in A375 Human Melanoma Cells
Authors: Kotowski K, Supplitt S, Wiczew D, Przystupski D, Bartosik W, Saczko J, Rossowska J, Drąg-Zalesińska M, Michel O, Kulbacka J.
DOI: https://doi.org/10.21873/anticanres.14232, 2020
Published in: Anticancer Research
Tags: Immunooncology and oncology, B-raf kinase, 3PO, A375, BRAFV600E, Melanoma, PFKFB3, inhibitor, apoptosis, glucose uptake, cycle arrest
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TP3, an antimicrobial peptide, inhibits infiltration and motility of glioblastoma cells via modulating the tumor microenvironment
Authors: Ying-Fa Chen, Po-Chang Shih, Hsiao-Mei Kuo, San-Nan Yang, Yen-You Lin, Wu-Fu Chen, Shiow-Jyu Tzou, Hsin-Tzu Liu and Nan-Fu Chen
DOI: https://doi.org/10.1002/cam4.3005, 2020
Published in: Cancer Medicine
Tags: Immunooncology and oncology, neuroscience, cell mobility, glioblastoma multiforme, infiltration, TP3, tumor microenvironment, adhesion, migration, invasion, invasiveness, filopodia, cell attachment, metastasis, kinase, paxillin, RAS, AKT, ERK, JNK, p38
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Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
Authors: Paria Ali Pour, M. Cristina Kenney and Arash Kheradvar
DOI: https://doi.org/10.1161/JAHA.119.014501, 2020
Published in: Journal of the American Heart Association
Tags: Mitochondria and cell metabolism, mitochondrial transplantation, cardiac patients, cardiology, ATP, non-autologous, acute stress
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Specialized cytonemes induce self-organization of stem cells
Authors: Sergi Junyent, Clare L. Garcin, James L. A. Szczerkowski, Tung-Jui Trieu, Joshua Reeves, Shukry J. Habib
DOI: https://doi.org/10.1073/pnas.1920837117, 2020
Published: National Academy of Sciences
Tags: Stem cells, development, cell signaling, tissue formation, cell–cell communication, embryogenesis, Wnt
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Mesoporous silica nanoparticles functionalized with a dialkoxide diorganotin(IV) compound: In search of more selective systems against cancer cells
Authors: Diana Díaz-García, Lucia Sommerova, Andrea Martisova, Hana Skoupilova, Sanjiv Prashar, Tomas Vaculovic, Viktor Kanicky, Isabel del Hierro, Roman Hrstka, Santiago Gómez-Ruiz
DOI: https://doi.org/10.1016/j.micromeso.2020.110154, 2020
Published in: Microporous and Mesoporous Materials
Tags: Drug discovery, immunooncology & oncology,