“There are still many discoveries to be made on exogenous mitochondria biology, I’m convinced when we image different conditions and cell types with this platform, there will probably be valuable world-first discoveries.”

“Nanolive has developed a ‘one-click assay’ with no staining or centrifugation required, just a high-quality cell culture plus or minus the treatments of interest. This will allow us to learn orders of magnitude more about cell biology with less effort, less cost and greatly reduced infrastructure requirements relative to our legacy approaches.”

“In my career, with over 20 years of microscopy experience, I have seen many segmentation tools, but I can tell you this this segmentation analysis provided by Nanolive is the best segmentation I have ever seen.”

“Your data, with its dramatic resolution, provides a prime opportunity to measure the effect that a compound has on the morphology, pattern, and granularity of a cell, which we can use to predict the bioactivity of a compound. If we can identify compound-specific phenotypes, then we can screen other compounds for this phenotypic response and hypothesize that they function via a similar mechanism-of-action.”

Read the full testimonial here.

“Nanolive technology has been very useful in order to study the effect of our compounds on melanocytes with high resolution. Live-cell imaging allowed us to see how morphology and melanosomes accumulation changed in real time with the treatment, providing clear evidence of the in vitro efficacy of these compounds.”

“[With the LIVE Cell Death Assay], we can evaluate the kinetics of the cell death fraction. So using Nanolive’s microscope and the software, we can obtain fantastic cell death movies and data”

“I have worked with neurons for 7 years, and Nanolive images are by far the most detailed, high focus, live images I have ever seen”

“Continuous label-free live cell imaging is changing the paradigm for studying the mode of action of drugs. With the LIVE Cell Death Assay, the health of each single cell is determined at every single timepoint, ensuring temporal dynamics are quantified with the highest precision.”

“The Smart Lipid Droplet Assay is crucial for our research for several reasons: (1) we do not need to use any staining, which is less time-consuming, (2) we can follow the lipid droplet development throughout the process of cell differentiation, which has never been done before, and (3) we can perform multiple quantifications at the same time, which will improve the robustness of our results.”

“I believe that the Nanolive microscope together with the Smart Lipid Droplet Assay is a great option to analyze the dynamics of lipids in a “natural environment” as there is no need to add any labels or staining procedures which probably interfere with cellular processes. The software is easy to use and we are really looking forward to future experiments to further investigate impaired lipid homeostasis as a disease mechanism in several neurological disorders.”

“With Nanolive, we get much more information with less material and in completely non-invasive conditions. The possibility to interfere with LD metabolism by changing the incubation conditions and quantify the data with the Smart Lipid Droplet Assay “on-time” is just great.”

“The Nanolive technology is like putting on 3D glasses in a cinema – visualizing in depth cellular phenomena that are otherwise opaque and obscure.”

“When you work with NPs it is essential to characterize: the number of NPs that enter the cells, the site of accumulation and the morphological response of the cells over time. The CX-A is very nice for this as we can quantify how a cell population responds to NPs with different shapes and sizes, while retaining high single-cell resolution”.

Read the full testimonial here.

“Our preliminary results captured with the CX-A applied to COVID-19 research are striking and amazing because we have unprecedented details about the cell/cellular organization.”

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“The CX-A allows us to characterize the different effects of drugs on live cells in-vitro, in high resolution and with data that can later be easily analyzed.”

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“We want to understand how various electromagnetic fields affect the remodeling of the cytoskeleton in real-time with all associated physiological events and Nanolive imaging is an excellent commercial solution for this. We want to use our 3D Cell Explorer-fluo to monitor morphological changes in cells in real time.”

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“One of the major advantages of Nanolive imaging is that we can observe contact points between cells, migration patterns and different morphologies, all in an unperturbed system.”

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“We use our 3D Cell Explorer in all of our on-going projects because it allows us to easily look at the mitochondria without the artifacts of staining or phototoxicity.”

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“The Nanolive 3D Cell Explorer is a powerful tool to measure morphologic and functional features of live stem cells.”

“This is an excellent technology that allow us to visualise cellular compartments and their rapid dynamics live without the need for staining/ florescence. In combination with advances in segmentation, it will open the door for unprecedented quantitative biology at high temporal resolution.”

“It is amazing to be able to directly visualize the cell in its natural media, without labeling and in 3D. This allowed us to monitor the cell in 3D while sampling part of the cytoplasm and analyzing it using mass spectrometry, thus achieving quantitation on a subcellular space, possibly the first in the world to do so.

“Actelion researchers were among the first to explore the potential of the Nanolive 3D Cell Explorer in cell biological applications within the pharmaceutical industry. We were very happy that the system is so easy to set-up (plug-and-play) and we are still amazed by the beautiful images it generates.

” […] It is like a window opened on a new world.”  […] “What you did is fantastic. Your microscope is a great achievement. […] You bring a new way to see and to analyze cells. […] You give us the opportunity to read nature with an other physical quantity: the density.

“The 3D Cell Explorer enables us to study chromatin condensation and nanoparticle uptake in live cancer cells, which wouldn’t be possible with other methods. We are extending this work now to different cancer cell lines to explain variations seen in response to therapy.”

“It is absolutely fantastic! Its ease of operation, intuitive nature, compact size, rapid imaging and no need for stains make this a system I would certainly recommend and it would certainly support many different kinds of research.” [learn_more

Read the full story from Dr. Chrzanowski here!