Label-free Cell Biology in 3D

Cell biology with label-free 3D live cell imaging




Advancing cell biology with the 3D Cell Explorer – a label-free live microscope

Cell biology researchers can discover more about the behavior of cells with Nanolive’s microscope – the 3D Cell Explorer. This microscope offers the best contrast, resolution and versatility that a label-free microscopic approach can offer today, coupled to unmatched time resolution. Last but not least, it’s 3D.

Please have a look at our stunning cell biology material: stem cells as never seen before!

Long Term Live Imaging of Mouse Embryonic Stem Cells for 15 hours 

Long Term Live Imaging of Mouse Embryonic Stem Cells for 34 hours

Long-term imaging of fine cellular dynamics in cell biology

One of the biggest challenges in cell biology is long-term imaging of fine cellular dynamics. In particular, the 3D Cell Explorer generates no phototoxicity as it injects in the sample ~100 times less energy (~0.2 nW/µm2) than light sheet microscopes (~1nW/µm2) that are the reference in the matter and used in cell biology. With a resolution of 195nm (higher than standard confocal) it enables high resolution and high-frequency imaging even with sensitive cell biology material, such as stem cells*.

Unique dynamics in cell biology structures can now be clearly analyzed, such as mitochondria, lipid droplets, filopodia, dendrites, axons, nucleus and nucleoli etc.

With Nanolive’s living cell imaging tool, especially designed for cell biology, it is possible to perform endless live imaging at the maximal acquisition speed (1 image per 1.7 seconds). 

This certainly exceeds any living cell imaging capabilities of any other microscope and will revolutionize livig cell imaging in cell biology studies.

(*plus, the next generation holotomographic technology used to make these movies is sold at a fraction of the cost of a standard confocal)

Long Term Live Imaging of Mouse Embryonic Stem Cells for 9,5 hours! Part 1

Long Term Live Imaging of Mouse Embryonic Stem Cells for 48 hours! Part 2

Cell Biology Webinar

Cell Biology Webinar: Long-term 3D imaging and quantification of stem cell dynamics

Dr. Mathieu Frechin, Head of Quantitative Biology at Nanolive introduces you to our holotomographic microscopy and its implications in long-term imaging of stem cells in the area of cell biology. He outlines the advantages that Nanolive’s holotomographic technology has to study sensitive cell lines. In particular, the following topics are being covered:

  • Challenges in live cell imaging of stem cells
  • State of the art of label-free microscopy for cell biology
  • Highlights of our holotomographic approach on stem cell research presenting movies and interesting biological dynamics.

Cell Biology the 3D Cell Explorer-fluo

Cell Biology: Live FUCCI mESC imaged for over 48 hours 

The video shows live FUCCI mESC that were imaged for over 48 hours with Nanolive’s 3D Cell Explorer-fluo. A holotomographic image was taken every 14 seconds while a double channel (green and red) epifluorescence image was only taken every 30 holotomographic frames (7:30 minutes). The difference in acquisition rate was performed to reduce fluorescence related phototoxicity. 

*Please note that the latest fluorescence image is displayed over the following 30 holotomographic images until a new one is acquired, for visualization purpose. The mismatch between the two signals is due to this image processing choice.

The 3D Cell Explorer-fluo for cell biology applications

Multimodal complete solution for 3D live cell exploration

Combine 3D refractive index analysis with a fully integrated 3 channel fluorescence module to image your living cells as they are and as long as you want. Put chemical information into structural context for new biological insights.


Transform 2D fluorescence into 3D cell tomography: Identify cell organelles through fluorescence and monitor non-invasively their structures and dynamics in 3D and marker-free. Explore fluorescence (3 channels) and Digital Stains (8 channels) simultaneously.

Extended live cell imaging

Image your living cells as long as you need. Limit cell damages caused by fluorescent markers, bleaching and phototoxicity.

Cell Biology Application Note

Cell Biology Application Note: Stem Cell Growing and Filming with the 3D Cell Explorer

This application note focuses on the proper set up of the 3D Cell Explorer with its incubation system to perform long-time imaging (up to weeks) of mammalian stem cells, at high spatio-temporal resolution and in 3D.