The 3D Cell Explorer-fluo
Combine high quality holotomographic data with fluorescent markers Get a quoteCombine the Best of two Worlds
COMPLETE 3D SOLUTION
Combine high quality tomographic data with fluorescent markers
MULTIPLEXING
Explore multiple cell compartments
EXTENDED LIVE CELL IMAGING
Limit cell damages caused by fluorescent markers, bleaching and phototoxicity
At Nanolive, we focus on innovative product development at the highest standards to deliver a high precision and quality research device for our international customers.
Winner of Basel Life MipTec Product-Innovation Award 2017
Available Configurations
DAPI configuration: DAPI + FitC + TritC
Live Cell Imaging configuration: FitC + TritC + Cy5
All-in-one configuration*: DAPI + FitC + TritC / Cy5
*TritC and Cy5 excitation filters are exchangeable on channel 3
At Nanolive, we focus on innovative product development at the highest standards to deliver a high precision and quality research device for our international customers.
Revolutionary Technology
Multimodal Complete Solution for 3D Live Cell Exploration
Combine 3D refractive index analysis with a fully integrated 3 channel epifluorescence module to image your live cells as they are and as long as you want. Put chemical information into structural context for new biological insights.
2D section of RI map
Multi-channel epifluorescence
Overlay
3D reconstruction based on RI map
Multiplexing
Transform 2D fluorescence into 3D cell tomography: Identify cell organelles through fluorescence and monitor non-invasively their structures and dynamics in 3D & marker-free.
Co-localization for mitochondria
Co-localization for lysosomes
Co-localization for nuclear membrane
Co-localization for lipid droplets
Co-localization for plasma membrane
Co-localization for nucleus & nucleoli
Extended Live Cell Imaging
Image your live cells as long as you need. Limit cell damages caused by fluorescent markers, bleaching and phototoxicity.
Long-term imaging (11 hrs) of mouse pre-adipocytes. Mitochondria were labeled with mitTracker. A holotomographic image was taken every 15 seconds and a fluorescence image every 5 minutes.
Live FUCCI mESC imaged for over 48 hours
The video shows live FUCCI mESC that were imaged for over 48 hours with Nanolive’s 3D Cell Explorer-fluo. A holotomographic image was taken every 14 seconds while a double channel (green and red) epifluorescence image was only taken every 30 holotomographic frames (7:30 minutes). The difference in acquisition rate was performed to reduce fluorescence related phototoxicity.
*Please note that the latest fluorescence image is displayed over the following 30 holotomographic images until a new one is acquired, for visualization purpose. The mismatch between the two signals is due to this image processing choice.
Discover More
Combine high quality RI tomographic data and fluorescence markers
You want to extend your understanding of cell structures and cell mechanisms? Thanks to our 3D tomographic technology you can now have a powerful platform that combines RI information with fluorescence markers.
Go beyond fluorescence limitation
You are frustrated by the limitations of fluorescence markers? You would like to go further? Combine them with Nanolive’s digital staining and push the limits forward.
Achieve specific cell analysis while preserving cell life
Forget about fixation! With the 3D Cell Explorer-fluo you can now observe your cells as they are: alive, 3D and in motion!
Perform infinite live cell imaging
Limit cell damages caused by fluorescence markers, bleaching, and phototoxicity. The 3D Cell Explorer-fluo allows you to transform your chemical markers into physical ones which can be monitored for a limitless amount of time.
The Nanolive Top Stage Incubator
An okolab and Nanolive solution for live cell imaging
Top-Stage Incubator: Temperature; Humidity and CO2 (Manual mixing or Premixed)
This top-stage incubator ensures the highest temperature accuracy on sample temperature by controlling simultaneously up to 6 independent channels with a modern multichannel controller.
The system allows temperature, humidity and CO2 conditioning in a very user friendly manner.
The controller is equipped with an on-board memory for data logging and a mini-USB port for data download.
Nanolive 3D Cell Explorer-fluo is also compatible with the ibidi top-stage incubator. For more information follow this link: https://www.nanolive.ch/compatible-equipment/
Technical Features 3D Cell Explorer-ƒluo
| Illumination source | Holotomography (HT): Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2) Epifluorescence: CoolLED pE-300ultra, High speed switchable <100 μs, Lifetime >20’000 hours each channel |
|---|---|
| Resolution | 3D HT: x,y: 200 nm; z: 400 nm (3D image) Epifluorescence: rx,y: ~ 400 nm (2D image) |
| Field-of-view | HT: 90 × 90 × 30 μm Epifluorescence: 90 × 90 μm |
| Microscope Objective | Dry objective / 60x magnification / NA 0.8 |
| Channels | HT: Up to 7 simultaneous Epifluorescence: DAPI + FitC + TritC | FitC + TritC + Cy5 | DAPI + FitC + TritC / Cy5 |
| Imaging | HT: 3D Epifluorescence: 2D 4D time lapse: (RI + fluo) |
| Time resolution | HT: 0.5 fps 3D RI frame Epifluorescence: 3 fps each channel |
| Camera | USB 3.0 CMOS Sony IMX174 sensor / Quantum Efficiency (typical) 70 % (at 545 nm) / Dark Noise (typical) 6,6 e¯ / Dynamic Range (typical) 73,7 dB |
| Dimensions (width × depth × height in mm) |
3D Cell Explorer-fluo: 380 × 170 × 445 Fluorescence module: 77 × 186 × 162 |
| Weight | 12kg |
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