Nanolive’s LIVE T Cell Assay for multiparametric, non-invasive immuno-therapy analysis selects the best T cell therapies on living cells in a completely label-free fashion. The LIVE T Cell assay allows you to follow dynamic phenotypic changes in target cell populations and T Cell populations during a long period of time. In addition to providing novel information about target and T cell interactions at every timepoint, Nanolive’s imaging platform together with the LIVE T Cell Assay extracts continuous, multiplexed and unbiased data to reveal how T Cells find, bind, stress and kill their targets.

Are your T cells actively searching for their target cells? Use T cell speed and distance to target to find out and compare how your experimental treatment(s) impact the duration of this phase.

Compare how drug treatments or Chimeric Antigen Receptor (CAR) engineered T cells perform against their targets in vitro by calculating the duration of the binding phase.

Evaluate when your T cells start affecting your target cells by monitoring changes in the internal structure/composition of your target cells through granularity. This texture metric is unique to holotomographic imaging thanks to the richness of information contained in refractive index images.

Identify the precise moment your T cells start to kill their targets – whether it be within minutes or hours. Access unique dynamic information; when is the fastest killing phase and when do T cells start to slow down (i.e., become exhausted)?

Once your cells have killed, are they able to kill again and again? Effective serial killing is a desirable trait for candidate drugs and we offer a means of calculating it.

Label-free AI-assisted segmentation of target cells and T cells in co-culture

The LIVE T Cell Assay offers a unique opportunity to understand T cell/target cells interactions over time, at both the single-cell and population level. Nanolive’s label-free live cell imaging captures detailed, multiplexed, and texturally rich images of the entire T cell response process: find, bind, stress and kill.

Only Nanolive’s rich images can achieve a clear segmentation of T cells and their targets without using any destructive labels.

Complete phenotypic profiling and novel quantitative metrics

The Live T Cell Assay offers complete phenotypic profiling for both cell populations plus additional and novel metrics specific to T cell and target cell interactions.

Technical Note

LIVE T Cell Assay – Analyze the whole process

The T cell response toward target cells can be divided into five phases: find, bind, stress, kill, and (sometimes) serial kill. Nanolive’s label-free live cell imaging captures detailed, multiplexed, and texturally rich images of the entire process, and thus, offers a unique opportunity to understand T cell and target cell interactions over time, at both the single-cell and population level. The richness of the data though poses a considerable analysis challenge. Different cell types must be identified, segmented, and meaningful metrics extracted if significant conclusions are to be drawn. This technical note explores these challenges, and presents Nanolive’s cutting edge, AI-assisted solution, the LIVE T Cell Assay. It also includes a full use case study where the LIVE T Cell Assay is used to quantify the effect a bispecific antibody has on T cell and cancer cell dynamics.

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Nanolive's automated imaging platform

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