Scientific Publications

Authors: Anna Szewczyk, Jolanta Saczko, Julita Kulbacka

DOI: https://doi.org/10.1016/j.bioelechem.2020.107592, 2020 

Published in: Bioelectrochemistry

Tags: rhabdomyosarcoma, muscle cells, calcium ions, electroporation, caspase 3, apoptosis

Authors: Sara Metwally, Sara Ferraris, Silvia Spriano, Zuzanna J. Krysiak, Łukasz Kaniuk, Mateusz M. Marzec, Sung-Kyun Kim, Piotr K. Szewczyk, Adam Gruszczyński, Magdalena Wytrwal-Sarna, Joanna E. Karbowniczek, Andrzej Bernasik, Sohini Kar-Narayan, Urszula Stachewicz

DOI: https://doi.org/10.1016/j.matdes.2020.108915, 2020 

Published in: Materials & Design

Tags: surface potential, Kelvin probe force microscopy, zeta potential, cells, adhesion, mineralization, CX-fluo, bone regeneration, interaction, morphology, fibers, osteoblast, MG63

Authors: Zahady D. Velásquez, Sara Lopez-Osorio, Learta Pervizaj-Oruqaj, Susanne Herold, Carlos Hermosilla, Anja Taubert

DOI: https://doi.org/10.1007/s00436-020-06744-x, 2020

Published in: Parasitology research

Tags: Besnoitia besnoiti, apicomplexan parasites, coccidia, cell cycle arrest, CX-fluo, histone H3 S10, cell lysis, lysis, gondii, besnoiti, tachyzoites, PCNA

Authors: E. Ouni, C. Bouzin, M.M. Dolmans, E. Marbaix, S. Pyr dit Ruys, D. Vertommen, C.A. Amorim

DOI: https://doi:10.1093/humrep/deaa100, 2020

Published in: Human Reproduction

Tags: Ovary, extracellular matrix, hormone replacement therapy, CX, aging, fertility preservation, mechanobiology, elastic matrisome, matrisome, collagen, elastin, EMILIN-1, fiblilin-1, glycosaminoglycan, GAGs, folicle, ECM

Authors: Kuan-Chou Chen, Chang-Rong Chen, Chang-Yu Chen, Kai-Yi Tzou, Chiung-Chi Peng, Robert Y. Peng

DOI: https://doi.org/10.21873/anticanres.14232, 2020

Published in: Anticancer Research

Tags: Oncology and immunooncology, cell metabolism, CX, PC-3 Pca, bicalutamide, Bic, rat mesangial cell, RMC line, mitochondrial dysfunction, HIF-1, oxygen consumption rate, OCR, mitochondria, pgrostate cancer, Pca, cardiovascular damage, renal damage, lactate cehydrogenase, ROS, lysosome, BCL2, caspase-3

Authors: David Scieszka, Yi-Han Lin, Weizhong Li, Saibyasachi Choudhury, Yanbao Yu, Marcelo Freire

DOI: https://doi.org/10.21873/anticanres.14232, 2020

Published in: bioRxiv

Tags: Immunology, single cell characterization, Immunology, single cell characterization, CX, HL60, HL-60, ATCC, NETs, neutrophils, extracellular DNA, genomics, proteomics, NETOME, inflammation, infection, antimicrobial

Authors: Chien-HanYuan, Yi-LingMa, Po-ChangShih, Chao-TingChen, Shu-YuCheng, Chieh-YuPan, Yen-HsuanJean, Yih-MinChu, Sung-ChunLin, Yu-ChengLai, Hsiao-MeiKuo

DOI: https://doi.org/10.21873/anticanres.14232, 2020

Published in: Biochemical Pharmacology

Tags: Immunooncology, oncology, cell metabolism, OS MG63, osteosarcoma, intrinsic apoptosis, TP3, antimicrobial peptide, mitochondria, caspase, blebbing, shrinkage, nuclear fragmentation, chromatin condensation, apoptopodia, ROS, acidification, fission, fusion

This Feature Application shows the enormous potential of Nanolive technology for observing stem cells in 3D for long periods of time, at unprecedented spatial and temporal resolution (case study 1). We use our technology to capture all the phases of mitosis (case study 2) and the dynamics of stress fiber formation (case study 3), in unparalleled detail. We then showcase our most ground-breaking contribution to this research field, monitoring the sub-cellular morphological changes that occur during stem cell differentiation. We focus on neural differentiation at the single cell (case study 4) and population level (case study 5), before examining the temporal evolution of osteogenic (case study 6) and chrondrogenic differentiation (case study 7) in high resolution.

This Feature Application highlights the huge potential of Nanolive cell imaging for metabolic studies. We begin by proving that mitochondria and LDs have specific RI signals that allow them to be identified and mapped, in 3D (case study 1). We show that it is possible to image fission-fusion dynamics in mitochondria (case study 2), induce and detect mitochondrial dysfunction (case study 3) and then restore mitochondrial function by the addition of a specific drug (case study 4). Finally, we show that it is possible to quantify LD dynamics in unperturbed (case study 5) and drug-perturbed conditions (case study 6).

In this application note, we begin by examining the interactions between t-cells, dendritic cells, and macrophages (case study 1). We then image t-cells responses to cancer cells (case study 2) and the interactions that occur between t-cells and macrophages (case study 3). We show Nanolive imaging can be used to monitor the response of cancer cells to promising antibody drug conjugate (ADCs) products (case study 4) and novel bispecific antibodies (case studies 5 and 6). Finally, we finish with an example of a drug screen where we describe the phenotypic response of living cells to various kinase inhibitors that have shown promise as anti-cancer treatments (case study 7).