Smart Lipid Droplet Assay LIVE

The first smart digital assay to provide a push button solution

for analyzing lipid droplets dynamics, label-free

High precision segmentation of lipid droplets in cells label-free

The Smart Lipid Droplet Assay segments lipid droplets based on their unique refractive index values for optimal results without the addition of stains or dyes.

It delivers 39 metrics for a complete quantitative analysis of LD morphology, content, and distribution, at the single cell scale and the population level.

In my career, with over 20 years of microscopy experience, I have seen many segmentation tools, but I can tell you this segmentation analysis provided by Nanolive is the best segmentation I have ever seen.

Dr. Jiansong Xie

Senior Scientist, from Amgen

Poster: Label-free lipid droplet quantification

Lipid droplets are dense lipid stores in the cytoplasm, that play a role in metabolic regulation. They are translucent and cannot be detected using traditional light microscopy without using dyes. However, they have a high refractive index, so can be detected label-free using Nanolive imaging, confirmed by correlative fluorescence imaging. The Smart Lipid Droplet Assay (SDLA) segments lipid droplets and cell boundaries automatically to quantify changes in lipid droplets over time.

Download the poster to read our new case study.

User-friendly push button assay to analyze lipid droplets dynamics 100% non-invasively

Make phototoxicity and photobleaching a thing of the past: with label-free imaging you can monitor your lipid droplet dynamics under your experimental conditions for as long as you need.

Nanolive has developed a ‘one-click assay’ with no staining or centrifugation required, just a high-quality cell culture plus or minus the treatments of interest. This will allow us to learn orders of magnitude more about cell biology with less effort, less cost and greatly reduced infrastructure requirements relative to our legacy approaches.

Dr. John Ferbas

Director, Department of Medical Sciences at Amgen

Complete profiling of lipid droplet dynamics at population, single cell and individual level

The Smart Lipid Droplet Assay delivers 39 metrics for a complete quantitative analysis of LD morphology, content, and distribution, at the single cell scale and at the population level.

SLDA Metrics

Technical Note

Technical Note SLD

Smart Lipid Droplet Assay

The Smart Lipid Droplet Assay has the potential to revolutionize medical research, by providing researchers access to an entire scale of lipid droplet (LD) dynamics that are unattainable using current techniques.

Nanolive imaging is label-free; no phototoxicity is generated and thus, there is no need to compromise between spatial and temporal resolution. The outcome? High content, multiplexed images with unparalleled sub-cellular resolution. Although LDs are easily identifiable by their high refractive index values, quantifying them, is not trivial, particularly if a high number of LDs are present, or they are closely clustered. This is why we have developed the Smart Lipid Droplet Assay the world’s first label-free image analysis solution for segmenting and quantifying LD dynamics.

Nanolive's automated imaging platform

The 3D Cell Explorer 96focus

Unlock the power of unlimited high content live cell analysis with the 3D Cell Explorer 96focus. Our unique approach enables analysis and re-analysis of label-free image data with our AI-powered digital assays. As always, the simple, autonomous workflow ensures that your precious time in the lab is used efficiently, and you can be confident that your samples are in good hands, whether you are imaging for an hour, or days.

  • Streamlined, automated workflows: save time and money
  • Long-term monitoring of cells label-free
  • High content, multiplexed, reliable live cell data
  • Fully integrated, cutting-edge digital analysis solutions


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