Stem cells, differentiation, regenerative medicine and cell therapyObserve stem cells in 3D over long periods of time at high spatial and temporal resolution with the 3D Cell Explorer Request a demo or quote
Capturing the deposition and early growth of bone that occur during osteogenic differentiation
Here, we use Nanolive’s 3D Cell Explorer 96focus to capture the morphological and physiological changes that occur during osteogenic differentiation.
The label-free nature of our technology allowed us to image the process from day 1 to day 13, in a non-perturbed state, and at high temporal resolution (1 image taken every 10 mins using the 5×5 gridscan mode).
MSCs were grown on fibronectin-coated dishes until 100% confluency was reached and then MSC osteogenic differentiation medium (C-28013, PromoCell) was added.
Read the related blog post here.
Stem cells differentiating into neuronal cells
This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.
Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s 3D Cell Explorer 96focus.
Read the related blog post here.
3D long-term live imaging of stem cells
Mouse Embryonic Stem Cells were imaged for 15 hours; 1 image every 15s. The cells are visibly healthy because they continue to divide during image acquisition.
The cells were imaged with the 3D Cell Explorer.
Download here our poster “Phototoxicity in live cell imaging: the evil within”.
High resolution imaging of mitosis in stem cells
In this video we can observe mitosis taking place in a living sample of human mesenchymal stem cells in spectacular detail. Cells were kindly provided by Promocell GmbH.
Read our detailed blogpost here.
Download here our poster “Investigating fine scale dry mass dynamics during mitosis using Nanolive cell imaging” and “Using Nanolive label-free cell imaging to examine nuclear rotation dynamics“.
Nanolive label-free live cell imaging has already shed light on many important topics in the field of stem cell research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.
Feature application: Stem Cells
In this Feature Application, we show how Nanolive’s technology can be used to: (1) observe MSCs in 3D over long periods of time at high spatial and temporal resolution; (2) capture mitotic division events in MSCs in unparalleled detail; (3) observe the dynamics of stress fiber formation and (4) visualize the morphological changes that occur during stem cell differentiation.
Watch our webinar: Characterization of stem cell differentiation dynamics with label-free live cell imaging
In this webinar, Dr. Emma Gibbin-Lameira, Communications Specialist at Nanolive will:
- discuss the unique challenges that stem cells pose to live cell imaging show how Nanolive’s new microscope, the 3D Cell Explorer 96focus, can overcome these difficulties
- present never-seen-before footage of mesenchymal stem cells undergoing neural differentiation
- discuss the dramatic phenotypic and morphological changes observed and the opportunities such research could offer
Please register here to view the webinar on-demand:
Mouse embryonic stem cells
Human mesenchymal stem cells incubated with neurogenic differentiation medium
Watch our webinar here
Nanolive imaging and analysis platforms
Swiss high-precision imaging and analysis platforms that look instantly inside label-free living cells in 3D
3D CELL EXPLORER 96focus
Automated label-free live cell imaging and analysis solution: a unique walk-away solution for long-term live cell imaging of single cells and cell populations
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence