Stem cells were isolated from the umbilical cord matrix and grown in culture for 14 days. At this stage most cells in the population are at the mature megakaryocyte stage and can start producing platelets in internal membranes present in their cytoplasm. In this footage (images taken at a frequency of one image per minute), we observe the formation and spontaneous release of platelets using Nanolive’s 3D Cell Explorer.
Being able to image such processes in living megakaryocytes and without the constraints imposed by fluorescent probes, opens the door towards more complicated mechanistic studies of in vitro platelet generation.
Nanolive would like to thank the team at the Transfusion Research Center (TReC) – Belgian Red Cross-Flanders for preparing the samples.
Read our latest news
Chondrogenic differentiation of mesenchymal stem cells imaged live, using Nanolive’s label-free, non-invasive imaging
Chondrogenic differentiation: from stem cell to cartilage Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types including osteoblasts (bone cells), neurones (nerve cells), chondrocytes (cartilage cells),...
Glioblastoma: the most aggressive brain cancer Glioblastoma multiforme (GBM) is an aggressive brain tumor that places among the most lethal of cancers. Unfortunately, most patients with GBMs die of their disease in less than a year and long-term survival prospects are...
Do you want to image stress fibers – the architecture of the cell – for unlimited periods of time at high frequency?
Stress fibers: what are they and why are they important? Stress fibers form the architecture of the cell. They are made of bundles of filaments composed from two strands of actin that are bound together by cross-linking proteins (see Figure 1). Their alignment,...
3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence