Single cell characteri­zation

Long-term label-free imaging of single cell phenotypes and cell organelles
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The only label-free live cell imaging tool that permits you to monitor cells for unlimited periods of time, without compromising spatial resolution. This allows to follow the development, behaviour, phenotype and function of single cells at the population level.

Long-term, label-free imaging of unperturbed single cells at a population level

In this video, an unperturbed population of 3T3-derived pre-adipocyte cells were imaged for 90 h using the 10×10 grid-scan mode on Nanolive’s CX-A (one image taken every 8.5 mins).

Watch as confluence increases from 9% to 60% over the course of the video (from 74 cells to 521) and marvel at the precision of the segmentation masks that make calculations like this possible.

Quantification of phenotypic variability at the population level

Phenotypic variation is a ubiquitous feature of all biological systems. It underpins complex, dynamic systems (e.g. immune responses) and biological processes (e.g. stem cell differentiation). Measuring changes in heterogeneity requires multiplex imaging platforms that maintain high spatial and temporal resolution, like Nanolive’s CX-A. Here, we provide the first quantitative analysis of phenotypic variability of single cells in a population of unperturbed cells.

Here, three cell metrics (cell area, dry mass, and compactness) are calculated for each cell, at every time point and visualized as relative frequency histograms. This approach allows to measure micro-heterogeneity in a population and to compare the variance that exists between traits.

Multiplexed organellular imaging

Nanolive’s high resolution allows the behaviour and dynamics of multiple cell organelles to be measured at the same time, without the addition of labels. In this video, we show close-up footage of the dynamics of five organelles (nucleus, mitochondria, lipid droplets, lysosomes, vesicles) and membrane protrusions in primary human keratinocytes.

 

Download here our poster “Quantifying dry mass dynamics in lipid droplets using Nanolive cell imaging”.

Capture and quantify novel biological phenomena including nuclear spinning

Researchers from EPFL have used Nanolive cell imaging to capture, and quantify, the dynamic behaviour of cellular organelles. Their findings, published in leading journal PLOS Biology, include an in-depth, quantitative analysis of the dry mass dynamics of mammalian organelles, and nuclear spinning.

Scientific Publications

Nanolive label-free live cell imaging has already shed light on many important topics in the field of single cell characterization research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.

Feature Application

Application Note Single Cell Characterization

Feature application: Characterization of single cells

In this Feature Application, we showcase the enormous potential that Nanolive live cell imaging holds for single cell characterization. In the first case study, we analyze micro-heterogeneity at the population and the temporal level in unperturbed cells and in the second and third case studies we focus on cells where intracellular trafficking and respiration were experimentally perturbed.

Download our Feature Application Note >

Webinar

Watch our webinar: Label-free analysis of living cell populations reveals controlled phenotypic variation among single cells

In this webinar, Dr. Emma Gibbin-Lameira, Communications Specialist at Nanolive:

  • Compares the variability of isolated single cells with the variability of single cells in a large population to determine whether heterogeneity is driven by internal or external factors.
  • Examines how the variability of individual traits change over time in unperturbed cells.
  • Analyzes how cells respond to perturbations in intracellular trafficking and respiration to determine whether targeting different systems induces/reduces the phenotypic variation of single cells in a population.

Please register here to view the webinar on-demand:

Webinar

Watch our webinar: Label-free quantification of cell metabolism – focus on mitochondria and lipid droplets dynamics

In this webinar, Dr. Emma Gibbin-Lameira, Communications Specialist at Nanolive will:

  • Show time-lapse footage of mitochondrial swelling and dysfunction following perturbation.
  • Introduce a cell metric called granularity and discuss whether changes in this measurement could be a signal of stress preceding cell death.
  • Present a fully quantitative analysis of lipid droplet dynamics in unperturbed cells, starting at the population level and finishing at level of an individual lipid droplet. Correlations between lipid droplet characteristics and other cell morphological traits (e.g. dry mass) will also be featured for the first time.

Please register here to view the webinar on-demand:

Video library

Transport of melanin granules in melanocytes

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Structure of melanin granules in melanocytes

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Stress fibers under Nanolive 3D Cell Explorer-fluo

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Nanolive imaging: Failed mitosis and apoptosis by multinucleated mouse pre-Adipocyte

Primary cilia in Xenopus cells

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3T3-derived pre-adipocyte cells under inhibition of respiration by Rotenone

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Norepinephrine effect on 3T3-derived pre-adipocyte cells

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Mitochondria in preadipocytes

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Label-free Live Cell Imaging: Living Macrophages Marker-free imaged for 24 hours

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Label-free live cell imaging of Human Mesenchymal Stem Cells in vivo: The Cytoskeleton

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Label-free live cell imaging of Mesenchymal Stem Cells undergoing mitosis

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Protrusions in Human Umbilical Vein Endothelial Cells with Nanolive imaging

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Label-free live cell imaging of macropinocytosis (cell drinking)

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VPA effects on mitochondrial morphology and LD behaviour

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Compare Nanolive's microscopes

CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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3D Cell Explorer front view

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging

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