Scientific Publications

The Live T Cell Assay promises to accelerate the advancement of basic cell biology and of novel, safe, and effective immunotherapies. The goal of this application note is to show some of the potential uses of the Live T Cell Assay and hopefully, inspire research that will increase the success rate of anticancer drug development. To this end, we present the first in-depth investigation of bispecific antibodies using the Live T Cell Assay.

This Feature Application shows the huge potential that Nanolive imaging holds for the drug discovery process, where cytotoxicity remains one of the major causes of drug withdrawal, and there is an urgent need for reliable and time-saving assay workflows. Our first case study features an in-depth investigation of the dynamics of cell death following exposure to seven cell death stimuli: extreme shifts in external pH, thermal stress, phototoxicity, oxidative stress, Shikonin, Ebastine, and Taxol. Our second case study shows that we can quantify drug-dependent and dose-dependent effects in 96-well plate format.

Authors: Paola Bonaventura; Vincent Alcazer; Virginie Mutez; Laurie Tonon; Juliette Martin; Nicolas Chuvin; Emilie Michel; Rasha E. Boulos; Yann Estornes; Jenny Valladeau-Guilemond; Alain Viari; Qing Wang; Christophe Caux; Stéphane Depil

https://doi.org/10.1126/sciadv.abj3671, 2022

Published in: Science Advances

Tags: adaptive immunity; Human endogenous retroviruses; cancer vaccine; immunotherapy; solid tumors

Authors: Saeid Moghassemi; Arezoo Dadashzadeh; Paulo Eduardo Narcizode Souza; Ricardo Bentes Azevedo; Christiani A. Amorim

https://doi.org/10.1016/j.pdpdt.2021.102555, 2021

Published in: Photodiagnosis and Photodynamic Therapy

Tags: Photodynamic therapy; cancer therapy; photosensitizer; ovarian transplantation; leukemia; phthalocyanine; photoimmunotherapy

Authors: Ana Luisa Palacios-Acedo; Soraya Mezouar; Diane Mège; Lydie Crescence; Christophe Dubois; Laurence Panicot-Dubois

https://doi.org/10.3389/fonc.2021.704945, 2021

Published in: Frontiers in Oncology

Tags: pancreatic tumors; thrombosis; clopidogrel; aspirin; P2Y12 receptor

Authors: Francesca Carriero; Carolina Martinelli; Fabio Gabriele; Giulia Barbieri; Lisa Zanoletti; Gloria Milanesi; Claudio Casali; Alberto Azzalin; Federico Manai; Mayra Paolillo; Sergio Comincini

https://doi.org/10.3390/jpm11100942, 2021

Published in: Journal of Personalized Medicine

Tags: oxidative stress; phyto-therapy; astrocytoma; glial tumor

Authors: Łukasz Zadka; Igor Buzalewicz; Agnieszka Ulatowska-Jarża; Agnieszka Rusak; Maria Kochel; Ireneusz Ceremuga; Piotr Dzięgiel

https://doi.org/10.1016/j.ajpath.2021.08.005, 2021

Published in: The American Journal of Pathology

Tags: digital holographic tomography; extracellular vesicles; Evs; colorectal cancer; ulcerative colitis

Authors: Deborah Fanfone; Zhi Chong Wu; Jade Mammi; Kevin Berthenet; David Neves; Kathrin Weber; Andrea Halaburkova; François Virard; Félix Bunel; Hector Hernandez-Vargas; Stephen WG Tait; Ana Hennino; Gabriel Ichim

https://doi.org/10.7554/eLife.73150, 2022

Published in: eLife

Tags: metastasis; invasion; anoikis; cancer; motility; evasion; resistance

Authors: Rui Zan; Hao Wang; Weijie Cai; Jiahua Ni; Bérengère J. C. Luthringer-Feyerabend; Wenhui Wang; Hongzhou Peng; Weiping Ji; Jun Yan; Jiazeng Xia; Yang Song; Xiaonong Zhang

https://doi.org/10.1016/j.bioactmat.2021.07.026, 2021

Published in: Bioactive Materials

Tags: Biodegradable magnesium; hydrogen; tumor apoptosis; underlying mechanism; P53

Authors: A.A. Zhikhoreva; A.V. Belashov; A.B. Danilova; N.A. Avdonkina; I.A. Baldueva; M.L. Gelfond; T.L. Nekhaeva; I.V. Semenova; O.S. Vasyutinskii

https://doi.org/10.1016/j.jphotobiol.2021.112235, 2021

Published in: Journal of Photochemistry and Photobiology B: Biology

Tags: Digital holographic microscopy; photodynamic treatment; phase images; malignant cells; individual patients