Neuroscience

Long term non-invasive live cell imaging to investigate cellular neuroscience
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The non-invasiveness of Nanolive’s label- and phototoxicity-free technology means very sensitive cells such a neuronal cells can be imaged for long-term periods of time, without handling or experimentally-induced perturbations.

Primary cortical neuron differentiation: a timeline

During development, neurons build the processes (neurites) required for cell-cell communication. This requires several complex morphological changes including growth cone formation, axon specification and branching. Here we observe how neurons develop, over time, in high-resolution.

Unstimulated human cortical neuron 2 cells (hCN2) were imaged. Neurite stimulation media was then added and cells were re-imaged 20 h, 3 d, 9 d and 20 d post-media addition. All images were acquired at a rate of one
image every 6 seconds using Nanolive’s 3D Cell Explorer-fluo. The high temporal resolution of Nanolive imaging enabled us to capture several novel cell behaviours (e.g. pulsing), which we quantify. 

For more information, read the related blog post here.

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Human mesenchymal stem cell differentiation into neurons

This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.

Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s CX-A.

Read the related blog post here.

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Human mesenchymal stem cell differentiation into neurons

This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.

Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s CX-A.

Read the related blog post here.

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Neuron on carpet of astrocytes

In this video, one image was taken every 20 secs for 20 mins, using Nanolive’s 3D Cell Explorer-fluo. Nanolive would like to thank Julie Nguyen and Lydia Danglot from the NeurImag Imaging Facility, located in the Institute of Psychiatry and Neurosciences of Paris for preparing the samples.

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Neural plasticity in live rat hippocampal neurons

In this video we can observe rat hippocampal neurons in culture.

The cells were at 7 DIV, when they already become “mature neurons”. In this short video we can clearly observe two distinctive phenomena characterizing the normal physiology of this beautiful cell type: vesicle migration and neuronal plasticity.

A special thanks for the beautiful sample goes to the lab of prof. Šuput’s group at the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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Neural plasticity in live rat hippocampal neurons

In this video we can observe rat hippocampal neurons in culture.

The cells were at 7 DIV, when they already become “mature neurons”. In this short video we can clearly observe two distinctive phenomena characterizing the normal physiology of this beautiful cell type: vesicle migration and neuronal plasticity.

A special thanks for the beautiful sample goes to the lab of prof. Šuput’s group at the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Request a quote or demo >

Scientific Publications

Nanolive label-free live cell imaging has already shed light on many important topics in the field of neuroscience research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.

Feature Application

Feature application: Neuroscience

This Feature Application shows the huge potential that Nanolive cell imaging holds for neurobiological research. Our first case study shows a timeline of the morphological changes undifferentiated primary cortical neurons undergo after exposure to neurite stimulation media. High precision segmentations are used to calculate cell metrics (e.g. volume, shape and dry mass) of interest. These calculations are directly linked to novel behaviours observed in individual neurons.

Download our Feature Application Note >

Webinar

Watch our webinar: Unlocking the mysteries of neurite growth in primary cortical neurones: a quantitative approach to live cell imaging

 

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive shows how label-free imaging can be used to capture and analyze the fine, dynamic details of neurite growth in unperturbed primary cortical neurons. Specifically, Dr. Gibbin shows:

  • a timeline of the morphological changes undifferentiated primary cortical neurones undergo after exposure to neurite stimulation media
  • high-precision segmentations that show it is possible to separate and quantify the responses (e.g. volume, shape, and dry mass) of neurites from the cell body
  • novel behavioural observations from individual neurons

Please register here to view the webinar on-demand:

Video library

Rat hippocampal neurons in culture. The purple colored video is digitally stained and in 3D

Stem cell differentiating into neuronal cells

Read the blogpost here.

Human mesenchymal stem cells differentiating into neuronal cells

Read the blogpost here.

Mitochondria trafficking in healthy hippocampal neurons.

Read the blogpost here.

Unstimulated hCN2 cells

Read the blogpost here.

hCN2 cells 20h after neurite growth stimulation media addition

Read the blogpost here.

hCN2 cells 3 days after neurite growth stimulation media addition

Read the blogpost here.

Primary cortical neuron 3 days after the addition of neurite stimulation media

Read the blogpost here.

Primary cortical neuron imaged with Nanolive 9 days after addition of neurite stimulation media

Read the blogpost here.

hCN2 cells 20 days after neurite growth stimulation media addition

Read the blogpost here.

Mitochondrial trafficking in neurons

Read the blogpost here.

Co-culture of neurons and migroglia

Read the blogpost here.

Compare Nanolive's microscopes

CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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3D Cell Explorer front view

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging

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