Phenotypic screening, cell differentiation, and neuronal networks
In this video, LUHMES neuronal precursor cells were stimulated to undergo differentiation into mature dopaminergic neurons. Cells were imaged using the 3×3 gridscan mode on the CX-A at an acquisition rate of 1 image every hour, for a total of 68 hours. By the end of the video, a mature neuronal-like network, containing extensive neurite outgrowths and growth cones is evident. And, if we zoom in, lipid droplets and mitochondria can be seen shuttling between neurons within the network. This research was conducted in collaboration with Dr. Urs Lüthi and Alexandre Peter from Swiss biotech company Idorsia.
Read our blogpost here.
Human mesenchymal stem cell differentiation into neurons
This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons. Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s CX-A.
Read the related blog post here.
Neurons on a carpet of astrocytes
In this video, one image was taken every 20 secs for 20 mins, using Nanolive’s 3D Cell Explorer-fluo. Nanolive would like to thank Julie Nguyen and Lydia Danglot from the NeurImag Imaging Facility, located in the Institute of Psychiatry and Neurosciences of Paris for preparing the samples.
Primary cortical neuron differentiation: a timeline
During development, neurons build the processes (neurites) required for cell-cell communication. This requires several complex morphological changes including growth cone formation, axon specification and branching. Here we observe how neurons develop, over time, in high-resolution. Unstimulated human cortical neuron 2 cells (hCN2) were imaged. Neurite stimulation media was then added and cells were re-imaged 20 h, 3 d, 9 d and 20 d post-media addition. All images were acquired at a rate of one image every 6 seconds using Nanolive’s 3D Cell Explorer-fluo. The high temporal resolution of Nanolive imaging enabled us to capture several novel cell behaviours (e.g. pulsing), which we quantify.
For more information, read the related blog post here.
Nanolive label-free live cell imaging has already shed light on many important topics in the field of neuroscience research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.
Feature application: Neuroscience
This Feature Application shows the huge potential that Nanolive cell imaging holds for neurobiological research. Our first case study shows a timeline of the morphological changes undifferentiated primary cortical neurons undergo after exposure to neurite stimulation media. High precision segmentations are used to calculate cell metrics (e.g. volume, shape and dry mass) of interest. These calculations are directly linked to novel behaviours observed in individual neurons.
Watch our webinar: Unlocking the mysteries of neurite growth in primary cortical neurones: a quantitative approach to live cell imaging
In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive shows how label-free imaging can be used to capture and analyze the fine, dynamic details of neurite growth in unperturbed primary cortical neurons. Specifically, Dr. Gibbin shows:
- a timeline of the morphological changes undifferentiated primary cortical neurones undergo after exposure to neurite stimulation media
- high-precision segmentations that show it is possible to separate and quantify the responses (e.g. volume, shape, and dry mass) of neurites from the cell body
- novel behavioural observations from individual neurons
Please register here to view the webinar on-demand:
Rat hippocampal neurons in culture. The purple colored video is digitally stained and in 3D
Primary cortical neuron imaged with Nanolive 9 days after addition of neurite stimulation media
Read the blogpost here.
Neural plasticity in live rat hippocampal neurons
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