Neuroscience

Long term non-invasive live cell imaging to investigate cellular neuroscience

The non-invasiveness of Nanolive’s label- and phototoxicity-free technology means very sensitive cells such a neuronal cells can be imaged for long-term periods of time, without handling or experimentally-induced perturbations.

Human Mesenchymal Stem Cell Differentiation into Neurons

This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.

Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s CX-A.

Read the related blog post here.

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Neural plasticity in live rat hippocampal neurons

In this video we can observe rat hippocampal neurons in culture.

The cells were at 7 DIV, when they already become “mature neurons”. In this short video we can clearly observe two distinctive phenomena characterizing the normal physiology of this beautiful cell type: vesicle migration and neuronal plasticity.

A special thanks for the beautiful sample goes to the lab of prof. Šuput’s group at the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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Neural plasticity in live rat hippocampal neurons

In this video we can observe rat hippocampal neurons in culture.

The cells were at 7 DIV, when they already become “mature neurons”. In this short video we can clearly observe two distinctive phenomena characterizing the normal physiology of this beautiful cell type: vesicle migration and neuronal plasticity.

A special thanks for the beautiful sample goes to the lab of prof. Šuput’s group at the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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Mitochondrial trafficking in neurons

In this video, neuronal mitochondria were stained using a fluorescent dye and then imaged for 10 mins with the 3D Cell Explorer-fluo at an acquisition frequency of 1 image every 10 secs.
Mitochondria can be seen being trafficked along axons, dendrites and within the cell body. They are also present in dendritic growth cones (actin-supported extensions of developing neurites).

Sample courtesy of Vanessa Morais and Rita Soares, iMM institute Lisbon.

Read our detailed blogpost here.

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Neuronal Mitochondria Dynamics

In this spectacular video of live neuron cells we can observe the dynamics and behavior of living and completely unperturbed mitochondria moving inside axons, dendrites and around the nucleus.

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Neuronal Mitochondria Dynamics

In this spectacular video of live neuron cells we can observe the dynamics and behavior of living and completely unperturbed mitochondria moving inside axons, dendrites and around the nucleus.

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Feature Application

Feature application: Neuroscience

This Feature Application shows the huge potential that Nanolive cell imaging holds for neurobiological research. Our first case study shows a timeline of the morphological changes undifferentiated primary cortical neurons undergo after exposure to neurite stimulation media. High precision segmentations are used to calculate cell metrics (e.g. volume, shape and dry mass) of interest. These calculations are directly linked to novel behaviours observed in individual neurons.

Download our Feature Application Note >

Webinar

Watch our webinar: Unlocking the mysteries of neurite growth in primary cortical neurones: a quantitative approach to live cell imaging

 

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive shows how label-free imaging can be used to capture and analyze the fine, dynamic details of neurite growth in unperturbed primary cortical neurons. Specifically, Dr. Gibbin shows:

  • a timeline of the morphological changes undifferentiated primary cortical neurones undergo after exposure to neurite stimulation media
  • high-precision segmentations that show it is possible to separate and quantify the responses (e.g. volume, shape, and dry mass) of neurites from the cell body
  • novel behavioural observations from individual neurons

Please register here to view the webinar on-demand:

Video library

Rat hippocampal neurons in culture. The purple colored video is digitally stained and in 3D

Stem cell differentiating into neuronal cells

Human mesenchymal stem cells differentiating into neuronal cells

Mitochondria trafficking in healthy hippocampal neurons.

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Unstimulated hCN2 cells

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Compare Nanolive's microscopes

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging             

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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