Microbiology & Biofilms
Long term non-invasive live cell imaging of microorganisms Compare Nanolive's ProductsFungi: Trichoderma reesei spore germination
In this footage, which was obtained during a demonstration organized by Dr. Matthias Steiger at the Technical University in Vienna, we observe the germination process in T. reesei at 30 °C. One image was acquired every 5 min for 2 h 45 min; a time frame sufficient to observe the initial formation of the germ tube. The high resolution of the images permit visualization of the growing membrane, internal structures and the formation of the wall, which occurs after 2 h 20 min..
Analysis of 3D colonies in bacteria
This image shows E.coli bacteria embedded in alginate beads (generated by Encapsulation Unit – Var J30, 40-70 microns diameter). The beads were mounted on a slide into Minimum Media (MM) diluted 1:4 with distilled water (plus 1% v/v Fumarate 100mM). The time-lapse imaging experiment was conducted using the 3D Cell Explorer with a standard top-stage incubator set to 37°C and 90% humidity for 3 hours, capturing images every 10 minutes.
Analysis of 3D colonies in bacteria
This image shows E.coli bacteria embedded in alginate beads (generated by Encapsulation Unit – Var J30, 40-70 microns diameter). The beads were mounted on a slide into Minimum Media (MM) diluted 1:4 with distilled water (plus 1% v/v Fumarate 100mM). The time-lapse imaging experiment was conducted using the 3D Cell Explorer with a standard top-stage incubator set to 37°C and 90% humidity for 3 hours, capturing images every 10 minutes.
Bacteria in tissues and cell samples
Rehydrated paraffin embedded bee gut sections (5 µm) imaged with the 3D Cell Explorer. The panoramic view of the whole tissue section (more than 400 micrometers) was obtained by stitching multiple acquisitions using a free online software. Like human intestine, bee gut is colonized by bacteria population. Most of the gut microbial community resides in the ileum.
Macrophages phagocytosing E.coli
Phagocytosis Assay Kits by PromoCell were used to test the viability and cellular functioning of the macrophages (video 2 & 3). E.coli particles, visible as small ellipsoid particles, are trapped by the cells, transported and lysed. This system can be used to provide a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like Receptor (TLR) ligands.
Read our detailed blogpost here.
Macrophages phagocytosing E.coli
Phagocytosis Assay Kits by PromoCell were used to test the viability and cellular functioning of the macrophages (video 2 & 3). E.coli particles, visible as small ellipsoid particles, are trapped by the cells, transported and lysed. This system can be used to provide a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like Receptor (TLR) ligands.
Read our detailed blogpost here.
Scientific Publications
Nanolive label-free live cell imaging has already shed light on many important topics in the field of microbiology and biofilms research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.
Video library
Bacteria growing in alginate beads
Pap-smear analysis
Fungi: Aspergillus niger spore germination
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3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
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Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence
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Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations