Microbiology & Biofilms

Long term non-invasive live cell imaging of microorganisms
Compare Nanolive's Products

Fungi: Trichoderma reesei spore germination

In this footage, which was obtained during a demonstration organized by Dr. Matthias Steiger at the Technical University in Vienna, we observe the germination process in T. reesei at 30 °C. One image was acquired every 5 min for 2 h 45 min; a time frame sufficient to observe the initial formation of the germ tube. The high resolution of the images permit visualization of the growing membrane, internal structures and the formation of the wall, which occurs after 2 h 20 min..

Request a quote or demo >

 

Analysis of 3D colonies in bacteria

This image shows E.coli bacteria embedded in alginate beads (generated by Encapsulation Unit – Var J30, 40-70 microns diameter). The beads were mounted on a slide into Minimum Media (MM) diluted 1:4 with distilled water (plus 1% v/v Fumarate 100mM). The time-lapse imaging experiment was conducted using the 3D Cell Explorer with a standard top-stage incubator set to 37°C and 90% humidity for 3 hours, capturing images every 10 minutes.

Request a quote or demo >

 

Analysis of 3D colonies in bacteria

This image shows E.coli bacteria embedded in alginate beads (generated by Encapsulation Unit – Var J30, 40-70 microns diameter). The beads were mounted on a slide into Minimum Media (MM) diluted 1:4 with distilled water (plus 1% v/v Fumarate 100mM). The time-lapse imaging experiment was conducted using the 3D Cell Explorer with a standard top-stage incubator set to 37°C and 90% humidity for 3 hours, capturing images every 10 minutes.

Request a quote or demo >

 

 

Bacteria in tissues and cell samples

Rehydrated paraffin embedded bee gut sections (5 µm) imaged with the 3D Cell Explorer. The panoramic view of the whole tissue section (more than 400 micrometers) was obtained by stitching multiple acquisitions using a free online software. Like human intestine, bee gut is colonized by bacteria population. Most of the gut microbial community resides in the ileum.

Request a quote or demo >

 

 

Macrophages phagocytosing E.coli

Phagocytosis Assay Kits by PromoCell were used to test the viability and cellular functioning of the macrophages (video 2 & 3). E.coli particles, visible as small ellipsoid particles, are trapped by the cells, transported and lysed. This system can be used to provide a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like Receptor (TLR) ligands.

Read our detailed blogpost here.

Request a quote or demo >

 

 

Macrophages phagocytosing E.coli

Phagocytosis Assay Kits by PromoCell were used to test the viability and cellular functioning of the macrophages (video 2 & 3). E.coli particles, visible as small ellipsoid particles, are trapped by the cells, transported and lysed. This system can be used to provide a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like Receptor (TLR) ligands.

Read our detailed blogpost here.

Request a quote or demo >

 

 

 

Scientific Publications

Nanolive label-free live cell imaging has already shed light on many important topics in the field of microbiology and biofilms research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.

Video library

Bacteria growing in alginate beads

Pap-smear analysis

E.coli being engulfed through phagocytosis

Read our blogpost here.

 

Fungi: Aspergillus niger spore germination

E.coli embedded in alginate beads

Learn more >

Compare Nanolive's microscopes

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging             

Learn more >

3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

Learn more >

CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

Learn more >

Receive our latest news

Register for our newsletter!