Microbiology & host-pathogen interactions
Long term non-invasive live cell imaging of bacteria, viruses, fungi, and protozoa
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Nanolive’s high-resolution label-free imaging enables accurate and quantitative 4D spatio-temporal analysis of the behaviour of (and interactions between) micro-organisms. It offers the perfect tool for studying pathogens and their role in disease progression, which is critical for the successful development of vaccines and treatments.

Investigating COVID-19 infection

This video (images acquired every 10 mins for 20 h), shows the shocking impact that SARS-CoV-2 has on cell fate. Infected cells fuse with neighbouring cells, forming large multi-nucleated cells; a process known as syncytia formation. This footage, captured by post-doctoral researcher Dr. Blandine Monel, was kindly provided by Professor Olivier Schwartz from the Virus and Immunity lab at the Pasteur Institute in Paris.

Read the blogpost here.

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Testing a compound’s antimicrobial activity

In this video, we observe the response of Streptococcus pneumoniae to the endolysin, ClyJ-3. The disappearance of the bright spots in the refractive index images indicates that, as expected, most bacteria are killed by ClyJ-3. One image was taken every min for 2 h using the 3D Cell Explorer. This video was kindly provided by Prof. Hak-Kim Chan from the Faculty of Medicine and health at the University of Sydney (Australia).

Read the blogpost here.

Capturing virus-induced cytopathic effects

In this footage, obtained using Nanolive’s 3D Cell Explorer-fluo we observe the progression of GFP-labelled adenovirus in human bone osteosarcoma epithelial (U2OS) cells. Refractive images were taken every 15 s and fluorescence images were taken every 5 min using the FITC channel, for a total of 25 mins. This video was kindly provided by Prof. Clodagh O’Shea from the Salk Institute for Biological Studies (La Jolla, CA, USA). Read our interview with Prof. O’Shea here.

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Visualizing fungal spore germination

In this footage, which was obtained during a demonstration organized by Dr. Matthias Steiger at the Technical University in Vienna, we observe the germination process in Trichoderma reesei at 30 °C. One image was acquired every 5 min for 2 h 45 min using Nanolive’s 3D Cell Explorer. This time frame was sufficient to observe the initial formation of the germ tube. The high resolution of the images permit visualization of the growing membrane, internal structures and the formation of the wall, which occurs after 2 h 20 min.

Observing the progression of the intra-erythrocytic cell cycle of protozoans

To infect its host, the parasite Plasmodium falciparum must avoid macrophage detection, and degrade huge amounts of haemoglobin. This loss of haemoglobin is visible under the 3D Cell Explorer.

In this video, we observe the morphological changes induced by P. falciparum infection at different stages of the parasite’s life cycle.

Read more in our detailed blogpost here.

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Scientific publications

Nanolive label-free live cell imaging has already shed light on many important topics in the field of microbiology and host-pathogen research. To get inspired and learn how your research can benefit from our technology, we invite you to check out these scientific articles published by our clients.

Video library

Macrophages phagocytosing E. coli.

Read more in our blogpost here

Aspergillus niger spore germination in 3D

 

Mouse macrophages infected with Listeria monocytogenes.

Read more in our blogpost here

 

Toxoplasma gondii infection in mouse fibroblasts

 

 

Pneumonia bacteria phagocyted by neutrophils

Read more in our blogpost here

Human papillomavirus infection in HeLa cells

Read more in our blogpost here

Label-free detection of rhinovirus replication in 3D

Read more in our blogpost here

NETosis process in real time

Read more in our blogpost here

Toxoplasma infection in living fibroblast cells

Read more in our blogpost here

Compare Nanolive's Products

CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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3D Cell Explorer front view

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging

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