Webinars

Generating kinetic EC50 values using label-free cytotoxicity assays

In this webinar, Dr. Emma Gibbin-Lameira will show how Nanolive’s label-free, non-invasive imaging can be used to quantify dose-dependent responses of HeLa cells to Chloroquine.

Specifically, Dr. Gibbin-Lameira will show that:

  • Dose-dependent experiments can be performed in 96-well plate format over long periods of time (> 2 days), reducing the need for time and labor-intensive multiple fixed-point measurements and eliminating cytotoxic/cytostatic effects arising from the use of labels, dyes, or phototoxic damage
  • Metrics output from Nanolive’s proprietary software, EVE Analytics, can be used to generate statistically robust time-dependent EC50 values based on total dry mass; a metric unique to label-free imaging
  • Sub-cellular spatial resolution combined with high content image analysis can be used to investigate a drugs’ mechanism of action

Please register here to view the webinar on-demand

webinar EC50

Take a tea break with Emma and discover how to quantify cell cytotoxicity in 96 WP format label-free

A quick scientific tea break with Communication Specialist Dr. Emma Gibbin-Lameira, where a powerful cytotoxicity case study is presented, showing Nanolive’s unique live cell-based testing and high content analysis in 96-well plate.
Specifically, Dr. Gibbin-Lameira shown that:

  • Multiplexed quantitative descriptors of cell morphology and composition at both the population (field of view) and single cell level can be combined with high spatio-temporal images to evaluate drug responses in parallel, over long periods of time
  • It is possible to quantify dose-response effects of drugs on living cells in a multi-well set-up where different concentrations and different drugs can be tested in parallel.

Please register here to view the webinar on-demand

Tea time with Emma webinar

Label-free cytotoxicity assays: do different cell death stimuli show unique signatures in cell metrics?

In this webinar, Dr. Emma Gibbin-Lameira, Communications Specialist at Nanolive reveals how Nanolive imaging can be used to conduct real-time, automated cytotoxicity assays, without the contribution of photo and cytotoxic methodological artefacts. Specifically, she will:

  • Show high resolution, time-resolved footage of the dynamics of cell death following exposure to six cell death stimuli (extreme shifts in external pH, thermal stress, phototoxicity, oxidative stress, Shikonin and Ebastine)
  • Feature relevant cell metrics and/or close-ups of changes in sub-cellular morphology induced by each experimental condition
  • Discuss which cell metrics are best for distinguishing between different types of cell death

Please register here to view the webinar on-demand

Label-free cytotoxicity assays

Maximize the biological relevance of your data whilst shortening and simplifying your experimental workflow: EVE Analytics & the CX-A

In this webinar, Dr. Emma Gibbin-Lameira, Communications Specialist at Nanolive:

  • A step-by-step guide to setting-up an experiment and acquiring images with Nanolive’s automated microscope the CX-A, and creating publication-ready data and images using Nanolive’s new quantification solution, EVE Analytics
  • A case study showing how different stress stimuli (cytotoxicity vs. phototoxicity) invoke different types of cell death
  • An in-depth discussion of how cell metrics can be used to quantify different types of cell death

Please register here to view the webinar on-demand

Maximize the biological relevance of your data whilst shortening and simplifying your experimental workflow: EVE Analytics & the CX-A webinar photo

Label-free quantification of cell metabolism: focus on mitochondria and lipid droplets dynamics

In this webinar, Dr. Emma Gibbin-Lameira, Communications Specialist at Nanolive:

  • Shows time-lapse footage of mitochondrial swelling and dysfunction following perturbation.
  • Introduces a cell metric called granularity and discusses whether changes in this measurement could be a signal of stress preceding cell death.
  • Presents a fully quantitative analysis of lipid droplet dynamics in unperturbed cells, starting at the population level and finishing at level of an individual lipid droplet. Correlations between lipid droplet characteristics and other cell morphological traits (e.g. dry mass) are also be featured for the first time.

Please register here to view the webinar on-demand

Image Label-free quantification of cell metabolism: focus on mitochondria and lipid droplets dynamics

Label-free analysis of living cell populations reveals controlled phenotypic variation among single cells

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive:

• Compares the variability of isolated single cells with the variability of single cells in a large population to determine whether heterogeneity is driven by internal or external factors.
• Examines how the variability of individual traits change over time in unperturbed cells.
• Analyzes how cells respond to perturbations in intracellular trafficking and respiration to determine whether targeting different systems induces/reduces the phenotypic variation of single cells in a population.

Please register here to view the webinar on-demand

Thumbnail Single Cell Webinar

Unlocking the mysteries of neurite growth in primary cortical neurones: a quantitative approach to live cell imaging

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive shows how label-free imaging can be used to capture and analyze the fine, dynamic details of neurite growth in unperturbed primary cortical neurons. Specifically, Dr. Gibbin shows:

  • a timeline of the morphological changes undifferentiated primary cortical neurones undergo after exposure to neurite stimulation media
  • high-precision segmentations that show it is possible to separate and quantify the responses (e.g. volume, shape, and dry mass) of neurites from the cell body
  • novel behavioural observations from individual neurons

Please register here to view the webinar on-demand

Neuroscience Webinar thumbnail

Accelerating oncology drug discovery with label-free imaging

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive will discuss the advantages of using label-free live cell imaging in the drug discovery process and show how Nanolive’s automated live cell imaging solution the CX-A, can be used to:

  • observe unique drug-induced phenotypes that fluorescence microscopy cannot capture
  • record dynamic cellular responses to drug perturbations
  • automate in vitro screening studies.

Dr. Gibbin will present novel results from two experimental screens designed to test the phenotypic and morphological responses of pre-adipocyte and cancer cells to 8 inhibitors or modulators of kinase activity.

Please register here to view the webinar on-demand

Characterization of stem cell differentiation dynamics with label-free live cell imaging

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive will:

 

  • discuss the unique challenges that stem cells pose to live cell imaging show how Nanolive’s new microscope, the CX-A, can overcome these difficulties
  • present never-seen-before footage of mesenchymal stem cells undergoing neural differentiation
  • discuss the dramatic phenotypic and morphological changes observed and the opportunities such research could offer

Please register here to view the webinar on-demand