LIVE Cell Death Assay

The first push button and automated solution

for profiling cell health, death, apoptosis and necrosis, label-free

A push-button, automated solution for profiling cell health and death

LIVE Cell Death Assay

The LIVE Cell Death Assay (LCDA) provides first-in-class cytotoxicity analysis of dynamic label-free live cell data. Developed using state-of-the-art machine learning, the LCDA has been trained to discriminate not only living cells from dead cells, but also apoptosis from necrosis.

The LCDA has been created to be as user-friendly as possible: a single click of a button is required to launch the analysis. It delivers 13 viability and death-related metrics.

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Continuous label-free live cell imaging is changing the paradigm for studying the mode of action of drugs. With the LIVE Cell Death Assay, the health of each single cell is determined at every single timepoint, ensuring temporal dynamics are quantified with the highest precision.

Dr José M. Padrón

Full Professor, from Universidad de La Laguna

Differentiating cell health based on morphology

The assay recognizes whether cells are alive, apoptotic, or necrotic. When image acquisition is complete, a single click of a button is required to launch the analysis, which proceeds automatically without any user interaction.

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Complete quantitative metrics

The LIVE Cell death assay generates 13 novel metrics cover all aspects of cell health and death. Furthermore, the standard package metrics are included for each population (living, dead, apoptotic, necrotic).

LCDA Metrics

Technical Note

TN LIVE Cell Death Assay

LIVE Cell Death Assay

Nanolive’s high content, texturally rich, multiplexed label-free live cell data provide a unique opportunity to study cell death dynamics, which is why we developed the LIVE Cell Death Assay (LCDA), the first fully quantitative, push-button analysis solution for profiling cell health and death.

The LCDA has been trained using the latest machine learning techniques to recognize whether cells are alive (viable) or dead, to discern whether they died by apoptosis or necrosis,  and to quantify the kinetics of the cell death process.

Webinar “The LIVE Cell Death Assay: Drug discovery applications of cell health, stress, death, and recovery analyses”

Maximize the biological relevance of your data whilst shortening and simplifying your experimental workflow: EVE Analytics & the CX-A webinar photo

In this webinar, we explore the following drug discovery applications of Nanolive’s LIVE Cell Death Assay – all completely label-free:

Cell Death

  • Drug screening in a 96-well plate
  • Differentiating between necrotic and apoptotic cell death
  • Detecting therapy resistance

Cell Health, Stress and Recovery

  • Detecting cell stress before death
  • Investigating therapy resistance using the Health Cell Index and Refractive Index imaging
  • Monitoring cell recovery from the apoptotic pathway (anastasis)

Nanolive's automated imaging platform

The 3D Cell Explorer 96focus

Unlock the power of unlimited high content live cell analysis with the 3D Cell Explorer 96focus. Our unique approach enables analysis and re-analysis of label-free image data with our AI-powered digital assays. As always, the simple, autonomous workflow ensures that your precious time in the lab is used efficiently, and you can be confident that your samples are in good hands, whether you are imaging for an hour, or days.

  • Streamlined, automated workflows: save time and money
  • Long-term monitoring of cells label-free
  • High content, multiplexed, reliable live cell data
  • Fully integrated, cutting-edge digital analysis solutions


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CX-A with 96-WP from Nanolive

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