Nanolive is proud to announce a new publication in the journal Theranostics from users of the Nanolive’s 3D Cell Explorer in the Laboratory of Heavy Ion Radiation Medicine of Chinese Academy of Sciences in Lanzhou, China.
Mitochondrial metabolic abnormalities have been linked to resistance to radiotherapy due to radiation cytotoxicity in cancer cells. In aerobic conditions, an increased glycolysis and lactate production instead of the much more efficient oxidative phosphorylation are observed in cancer cells. This change in metabolism is known as the Warburg effect.
Sun and colleagues have studied the radiosensitization effects induced by transplantation of mitochondria from normal human astrocytic into glioma cells. The focus of their study was to identify the mechanism of free mitochondrial transfer into host cells via a NAD+-CD38-cADPR-Ca2+-endocytosis pathway. Their results show that starvation treatment led to a decreased Warburg effect and a recuperation of aerobic respiration. Mitochondrial transplantation into glioma cells could reduce resistance to radiotherapy.
The 3D Cell Explorer was used to obtain live images of the dynamic behaviour of endocytosis during starvation treatment and of the interaction between this process and mitochondria. Nanolive imaging has proven to be a method of choice in organelle dynamics research, due to its non-invasive and phototoxicity free imaging. Their full publication is available here!
Figure: Transplantation of isolated mitochondria into U87 cell through endocytosis
 C. Sun et al., “Endocytosis-mediated mitochondrial transplantation: Transferring normal human astrocytic mitochondria into glioma cells rescues aerobic respiration and enhances radiosensitivity,” Theranostics, vol. 9, p. 12, 2019.
 O. Warburg, F. Wind, and E. Negelein, “THE METABOLISM OF TUMORS IN THE BODY.,” J. Gen. Physiol., vol. 8, no. 6, pp. 519–30, Mar. 1927.
Read our latest news
What is the function of melanin? Melanin describes the group of natural pigments that is responsible for skin and hair pigmentation and photoprotection of the skin and eye. Deficiency in melanin can cause several disorders and diseases including vitiligo, deafness,...
We are delighted to announce a new publication featuring the 3D Cell Explorer, has been published in the scientific journal, Materials & Design. The study, led by Prof. Urszula Stachewicz at the AGH University of Science and Technology in Kraków, Poland, used...
One of the major advantages of Nanolive’s label-free imaging is that images can be acquired at high frequency, over long periods of time. This allows us to analyse the responses of cells to drug-perturbation with high temporal precision. Here, we observe how...
3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence