CD47 is a potent Don’t eat me signal that enables cancer cells to evade immune surveillance and killing by innate immune cells, such as macrophages.
By using bispecific antibodies with a high affinity targeting arm (against a tumor-associated antigen, or TAA) and an anti CD47 arm of an optimized low affinity, the Don’t eat me signal is blocked selectively on TAA-positive cells, leading to the effective engulfment and degradation of tumor cells by macrophages (left-hand part of the video).
Due to the absence of a tumor-specific targeting arm, a monovalent anti CD47 bsAb is unable to bind and block CD47, with tumor cells that can resist the attack of macrophages (right-hand part of the video).
Images were taken with Nanolive’s 3D Cell Explorer at a rate of one image every 30 seconds.
For more information on fully human CD47-blocking κλ-bodies, please visit Light Chain Bioscience.
Read our latest news
Next upcoming event
Next product launch - 26.06.2023
17 new scientific publications featuring Nanolive technology
Since the start of the year, 17 more papers have been published using Nanolive imaging, featuring an incredibly broad range of cell types; stem cells, adipocytes, neurons, osteoblasts, neutrophils, melanocytes, e. coli, primary synoviocytes, and several cancer cell...
Nanolive imaging confirms success of mesoestetic’s new treatment at the subcellular level
We are happy to see that Nanolive imaging has been used to confirm the success of another new therapy, this time treating melasma. In their latest paper “Depigmenting topical therapy based on a synergistic combination of compounds targeting the key pathways involved...
Nanolive microscopes

3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging

3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

CX-A
Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations