Is it possible to restore phagocytosis of cancer cells?

CD47 is a potent Don’t eat me signal that enables cancer cells to evade immune surveillance and killing by innate immune cells, such as macrophages.

By using bispecific antibodies with a high affinity targeting arm (against a tumor-associated antigen, or TAA) and an anti CD47 arm of an optimized low affinity, the Don’t eat me signal is blocked selectively on TAA-positive cells, leading to the effective engulfment and degradation of tumor cells by macrophages (left-hand part of the video).

Due to the absence of a tumor-specific targeting arm, a monovalent anti CD47 bsAb is unable to bind and block CD47, with tumor cells that can resist the attack of macrophages (right-hand part of the video).

Images were taken with Nanolive’s 3D Cell Explorer at a rate of one image every 30 seconds.

For more information on fully human CD47-blocking κλ-bodies, please visit Light Chain Bioscience.

Read our latest news

A new diagnostic role for Nanolive’s refractive index imaging?

A new diagnostic role for Nanolive’s refractive index imaging?

The refractive index of cellular structures is an intrinsic property that is used by Nanolive’s imaging and digital assays to analyse cells and organelles label-free. The authors of a newly published paper in Cells have innovatively applied this technology to monitor...

Nanolive microscopes

Get a quote >

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging             

Learn more >

3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

Learn more >

CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

Learn more >