EVE Analytics for the 3D Cell Explorer-fluo

Rediscover your cells with EVE Analytics’ unique, robust, and high-precision segmentation technique

EVE Analytics for 3D Cell Explorer-fluo

Thanks to EVE Analytics we can now offer precise cell segmentation of living cells for virtually unlimited periods of time. EVE Analytics can segment numerous single cells over thousands of images with no change in quality, regardless of the level of cell crowding/confluence in the image.

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Applications

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Quantifying changes in granularity (cell composition) during cell division

In this video, we show how granularity – a texture metric that describes a cell’s internal composition – changes during mitosis. Mesenchymal stem cells were imaged once every 15 secs for 50 mins. The cell in the top right of the video divides into two daughter cells at minute 38 in the video, which corresponds to the lowest granularity value. This example highlights the potential Nanolive imaging holds for investigating biological processes.

Quantifying the intracellular replication of pathogens inside their host cells

In this video, we quantify changing in the total dry mass that accompany the intracellular accumulation of bacteria in HeLa cells incubated with Staphylococcus aureus and imaged every 20 secs for 20 mins. Graph shows an accumulation of total dry mass as the bacteria replicate inside the cells. Images courtesy of Dr. Ana Eulalio from the University of Coimbra (Portugal).

The 3D Cell Explorer-fluo – a multimodal complete solution for 4D live cell imaging & fluorescence

 

3D Cell Explorer-fluo front view

Image: 3D Cell Explorer-fluo

  • Combine fully label-free imaging with correlative epifluorescence to cover a broad array of live cell imaging requirements for your lab, department, or institute
  • Rediscover your living cells through multiplexing: Nanolive datasets contain the simultaneous acquisition of several biological features and organelles – therefore capturing various pathways of cell biology in real-time
  • Extend Live Cell Imaging: image your live cells as long as you need. Limit cell damages caused by fluorescent markers, bleaching and phototoxicity
  • Visualize the invisible: the 3D Cell Explorer-fluo allows you to visualize what would be too small to be captured label-free, such as: proteins, ions (e.g. calcium), viruses, etc

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Technical Note

 

Image showing EVE Analytics precise segmentation

Nanolive’s label-free technology makes it possible to image cells for long periods of time, at high temporal resolution. The quantity and complexity of the images generated allows us to visualize biological processes in unprecedented detail, but also magnifies the challenges associated with image analysis. Manual image registration and analysis is impossible and so computer-aided processing must be used to harness data complexity. In this technical note, we introduce the key elements involved in cell segmentation, which are essential to understand the novelty of EVE Analytics (EA), Nanolive’s software solution for quantitative cell analysis. We then evaluate the performance of EA segmentation against fluorescence-based segmentation and compare how metrics produced by both approaches differ.

Click here to get EVE Analytics for the 3D Cell Explorer-fluo!

Read our latest news

11 Scientific publications featuring Nanolive live cell imaging

11 Scientific publications featuring Nanolive live cell imaging

April and May have been busy months for our clients with 11 new scientific publications since our last update featuring Nanolive live cell imaging. Most articles were either immuno-oncology & oncology or microbiology & biofilm-related, but there was a sprinkle...

Nanolive microscopes

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Image CX-A

CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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Image 3D CELL EXPLORER-fluo

3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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Image 3D CELL EXPLORER

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging

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