Nanolive is pleased to share a new publication in Nature Scientific Reports from our users in the Justus-Liebig University of Giessen .
Besnoitia besnoiti is an emerging parasite in Europe, responsible for bovine besnoitiosis, an illness causing a significant economic impact on cattle industry.
Similarly to T. gondii, already featured here, B. besnoiti also needs to scavenge cholesterol from its host cell in order to replicate. However, the mechanism used by B. besnoiti to obtain cholesterol is still controversial.
On this work, Silva and colleagues study the model of cholesterol salvage by analysing B. besnoiti infection of primary bovine endothelial cells in vivo.
Their results demonstrate that B. besnoiti can obtain cholesterol both via endogenous than exogenous uptake. This finding reinforces the theory that the successful B. besnoiti infections in primary bovine endothelial host cells rely on both the parasite and the cell type.
The 3D Cell Explorer- fluo was used to confirm the presence of abundant lipid droplet-like structures in B. besnoiti-infected cells. Images were later analyzed using STEVE software (Figure below).
The full publication is available here!
Figure 1. Live cell holographic tomography-based illustration of lipid droplets in non-infected and B. besnoiti- infected BUVEC.
 Silva, L. M. R. et al. Besnoitia besnoiti infection alters both endogenous cholesterol de novo synthesis and exogenous LDL uptake in host endothelial cells. Sci. Rep. 9, 6650 (2019).
Read our latest news
What is the function of melanin? Melanin describes the group of natural pigments that is responsible for skin and hair pigmentation and photoprotection of the skin and eye. Deficiency in melanin can cause several disorders and diseases including vitiligo, deafness,...
We are delighted to announce a new publication featuring the 3D Cell Explorer, has been published in the scientific journal, Materials & Design. The study, led by Prof. Urszula Stachewicz at the AGH University of Science and Technology in Kraków, Poland, used...
One of the major advantages of Nanolive’s label-free imaging is that images can be acquired at high frequency, over long periods of time. This allows us to analyse the responses of cells to drug-perturbation with high temporal precision. Here, we observe how...
3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence