Stem cells label-free characterization
Observe stem cells in 3D over long periods of time at high spatial and temporal resolution with the 3D Cell Explorer Compare Nanolive's productsThe only label-free live cell imaging tool that lets you observe stem cells in 3D, over long periods of time at high spatial and temporal resolution and capture dynamic events in unparalleled detail.
Capturing the deposition and early growth of bone that occur during osteogenic differentiation
Here, we use Nanolive’s CX-A to capture the morphological and physiological changes that occur during osteogenic differentiation.
The label-free nature of our technology allowed us to image the process from day 1 to day 13, in a non-perturbed state, and at high temporal resolution (1 image taken every 10 mins using the 5×5 gridscan mode).
MSCs were grown on fibronectin-coated dishes until 100% confluency was reached and then MSC osteogenic differentiation medium (C-28013, PromoCell) was added.
Read the related blog post here.
Stem cell differentiating into neuronal cells
This video shows the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.
Cells were grown for approx. 2 days in complete mesenchymal stem cell neurogenic differentiation medium before the differentiation process portrayed in this movie could be observed.
The cells in this footage were imaged for 20 hours every 4,5 minutes using a 5×5 grid and Nanolive’s CX-A.
Stem cell differentiating into neuronal cells
This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.
Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s CX-A.
Read the related blog post here.
Stem cell differentiating into neuronal cells
This video shows the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.
Cells were grown for approx. 2 days in complete mesenchymal stem cell neurogenic differentiation medium before the differentiation process portrayed in this movie could be observed.
The cells in this footage were imaged for 20 hours every 4,5 minutes using a 5×5 grid and Nanolive’s CX-A.
3D long-term live imaging of stem cells
Mouse Embryonic Stem Cells were imaged for 15 hours; 1 image every 15s. The cells are visibly healthy because they continue to divide during image acquisition.
The cells were imaged with the 3D Cell Explorer.
Download here our poster “Phototoxicity in live cell imaging: the evil within”.
High resolution imaging of mitosis in stem cells
In this video we can observe mitosis taking place in a living sample of human mesenchymal stem cells in spectacular detail. Cells were kindly provided by Promocell GmbH.
Read our detailed blogpost here.
Download here our poster “Investigating fine scale dry mass dynamics during mitosis using Nanolive cell imaging” and “Using Nanolive label-free cell imaging to examine nuclear rotation dynamics“.
High resolution imaging of mitosis in stem cells
In this video we can observe mitosis taking place in a living sample of human mesenchymal stem cells in spectacular detail. Cells were kindly provided by Promocell GmbH.
Read our detailed blogpost here.
Feature Application
Feature application: Stem Cells
In this Feature Application, we show how Nanolive’s technology can be used to: (1) observe MSCs in 3D over long periods of time at high spatial and temporal resolution; (2) capture mitotic division events in MSCs in unparalleled detail; (3) observe the dynamics of stress fiber formation and (4) visualize the morphological changes that occur during stem cell differentiation.
Webinar 1
Watch our Webinar:
Long-term 3D imaging and quantification of stem cell dynamics
- Challenges in live cell imaging of stem cells
- State of the art of label-free microscopy for stem cells
- Highlights of our holotomographic approach on stem cell research presenting movies and interesting biological dynamics.
Webinar 2
Watch our webinar:
Characterization of Stem cell differentiation dynamics with label-free live cell imaging
In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive will:
- discuss the unique challenges that stem cells pose to live cell imaging show how Nanolive’s new microscope, the CX-A, can overcome these difficulties
- present never-seen-before footage of mesenchymal stem cells undergoing neural differentiation
- discuss the dramatic phenotypic and morphological changes observed and the opportunities such research could offer
Please register here to view the webinar on-demand:
Video library
Long Term Live Imaging of Mouse Embryonic Stem Cells for 34 hours!
Long Term Live Imaging of Mouse Embryonic Stem Cells for 48 hours!
Live FUCCI mESC imaged for over 48 hours
Human Mesenchymal Stem Cell interacting with 3D hydrogel
Mitosis CD34+ Hematopoietic stem cells
Mitosis in Mesenchymal Stem Cells
Label-free live cell imaging of Mesenchymal Stem Cells undergoing mitosis
15 hours continuous imaging mouse Embryonic Stem Cells
Stem cell differentiation
Human stem cells 65h post incubation with neurogenic differentiation medium
Human stem cells incubated with neurogenic differentiation medium
High resolution live footage of the formation and spontaneous release of platelets
Stem cell differentiation into neuronal cells
hMSC incubated with neurogenic differentiation medium
hMSC incubated with neurogenic differentiation medium
Imaging stress fibers in Human Mesenchymal Stem Cells
Compare Nanolive's microscopes
3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence
CX-A
Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations